More PCR Stuff

#flo

• DNA Extraction
• Two main steps
  • Breaking the cells open chemically, by breaking the membrane of the cell. Some substance/method also has to break open the nucleus after the previous one is done. => (Of course, you could also acoustically, by ultrasonic waves to break, or mechanically, by like breaking breaking it)
    • The chemical usually used to do this is a detergent, like soap
    • Lipid bi-layer's hydrophobic tails becomes attracted to the detergent, and it gets ripped apart
    • Or, changing the pH of the solution may also be one method by which this could take place
  • Then, DNA is separated from other proteins & debris => This is usually done with either enzymes or using heat
• How the QuickExtract possibly work
  • A detergent breaks the outside lipid layer and the nucleus
  • Then, a protease chews up the enzyme and other random proteins
  • Finally, the whole thing's heated to make sure that the only thing left is the DNA